Quantitative determination of the total phenolic

The projections are obtained by decomposing the corresponding matrix. Then a residual is calculated and the calculation of the next component is based on this residual. The obtained results for equations need to be evaluated by using the test set.

Quantitative determination of the total phenolic

This article has been cited by other articles in PMC. Abstract Actaea spicata Linn. Ranunculaceae has been traditionally used for the treatment of various ailments such as rheumatism, inflammation, nerve diseases, lumbago, scrofula and chorea, but no systematic phytochemical and pharmacological work has ever been carried out on this potential plant.

Preliminary phytochemical screening showed presence of phenols and flavonoids in A. Thus, the present investigation was undertaken to estimate total phenols and flavonoids in methanol extract of A.

In vitro antioxidant activity was also evaluated in the methanol extract and ethyl acetate fraction using DPPH method. Ethyl acetate fraction was found to contain twice the content of flavonoids and phenols in comparison to methanolic extract, whereas phenolic content in methanol extract was approximately similar to ethyl acetate fraction.

A significant antioxidant activity, i. Finally, it was suggested that polyphenols are responsible for antioxidant activity of A. Actaea spicata, antioxidant activity, flavonoids, phenols Actaea spicata Linn.

A survey of ethnopharmacologic records reveals that the plant has been traditionally used in the treatment of rheumatism, inflammation, rheumatic fever, lumbago, scrofula, nervous disorders, chorea, and as emetic, expectorant, laxative, stomachic and purgative[ 1 — 3 ]. The plant has also been used in traditional systems of medicines of various countries for the treatment of snake bite, asthma, and externally for skin complaints.

Quantitative determination of the total phenolic

In some parts of Europe the powdered leaves, stems and flowers are used as an insecticide[ 4 ]. A thorough survey of literature revealed no pharmacological report on A.

Despite a long tradition of use for the treatment of various ailments, no systematic phytochemical and pharmacological work has ever been carried out on this potentially useful plant. Thus, the present investigations were planned with an objective to estimate total phenols and flavonoids in methanol extract of A.

Further, antioxidant activity was evaluated in methanol extract and ethyl acetate fraction of the plant using DPPH method. Dried roots of A. Identity of the plant was confirmed through Dr.

A new spectrophotometric enzymatic method for the determination of total phenol content in tea and wine has been developed. The method is based on the peroxidase-catalyzed oxidation, by hydrogen peroxide, of phenols to phenoxyl radicals, which can react with aromatic substrates to . Journal of Fruit and Ornamental Plant Research Vol. 14 (Suppl. 2), DETERMINATION OF PHENOLIC COMPOUNDS IN APPLES AND PROCESSED APPLE PRODUCTS. Quantitative determination of total phenols was done on the basis of a standard curve of gallic acid and linearity of the calibration curve was achieved between .

Folin Ciocalteu's reagent S. Fine Chemicals, MumbaiPotassium acetate S.

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Dried, coarsely powdered roots of A. The marc was air dried and extracted with methanol using a Soxhlet apparatus. The methanol extract was dried under reduced pressure using rotary vacuum evaporator Perfit, Ambalaand screened for different classes of phytoconstituents[ 6 ]. The methanol extract 25 g of A.

This procedure was repeated five more times. All the shakings of ethyl acetate were pooled and concentrated under reduced pressure. The ethyl acetate fraction 6. One ml aliquot of each dilution was taken in a test tube and diluted with 10 ml of distilled water. One ml aliquot of the sample was taken in a test tube and diluted with 10 ml of distilled water.

Absorbance of the sample was measured at nm against blank, i. Quantification was done on the basis of a standard curve of gallic acid. A standard curve of extinction against gallic acid concentration was prepared fig.Methods. The total phenolic content was determined quantitatively using the Folin Ciocalteu reagent, with Gallic acid as the standard.

The fluorescence characteristics of the powdered drug and successive extracts with and without chemical treatment during the day and under a UV light were recorded. Determination by the Folin method also gave low (– %) values although the results agreed satisfactorily with the content of phenolic acids obtained by dual-wavelength spectrophotometry (regression equation: y = ∙x + , where y is the content of phenolic acids determined by the developed method, x, the total content of.

Beta-damascenone,

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